Biol 26. Introductory Microbiolgy LabsRequired Reading:
1. Lab Manual: "Biology 26 Laboratory Manual" by Doris Spanggord
2. Bergey's Manual 3. Difco Manual: http://www.bd.com/ds/technicalcenter/inserts/difcoBblManual.asp
Key Safety Rules:http://facultyfiles.deanza.edu/gems/gilleskay/KeyLabSafetyRules.pdf
Laboratory Schedule - Summer 2009:
|
Wk
|
Date to be
Performed
|
Lab Exercise #
|
Subject
|
|
1
|
Mon, June 29
|
|
Orientation
to Lab
|
|
Tue, June 30
|
|
Introduction
to Microsocpy
Observation
of Living Organisms
|
|
Wed, July 1
|
|
Media
Preparation
Distribution
of Microorganisms in the Environment
|
|
2
|
Mon, July 6
|
|
Bacterial
Smear, the Simple Stain
The Negative
Stain
|
|
Tue, July 7
|
|
Capsule
Stain
The Acid
Fast Stain
DEMOS:
Endospore & Metachromatic Granule Stains
|
|
Wed, July 8
|
|
The Gram
Stain; Quiz #1
|
|
3
|
Mon, July 13
|
|
The
Bacterial Unknown
Isolation of
Pure Cultures (Lecture Exam #1)
|
|
Tues, July
14
|
|
Aerobic and
Anaerobic Culture Techniques
Catalase and
Oxidase Production by Bacteria
Reduction of
Nitrates
|
|
Wed, July 15
|
|
Action of
Bacteria on Simple Carbohydrates
Citrate Utilization
Hydrolysis
of Starch
|
|
4
|
Mon, July 20
|
|
Hydrolysis
of Gelatin
SIM Agar –
Determination of Motility, Indole and H2S Production
Urea
Hydrolysis
|
|
Tues, July
21
|
|
Review
|
|
Wed, July 22
|
|
Lab Exam
|
|
5
|
Mon, July 27
|
|
The MR – VP
Tests
API 20E Multiple
Test Strip
Selective
and Differential Media
|
|
Tues, July
28
|
|
Bacteria of
the Throat (Lecture Exam #2)
Bacteria of
the Skin
|
|
Wed, July 29
|
|
Viruses –
Bacteriophage Plaque Assay
Helminths
(Platyhelminths / Nematodes)
|
|
6
|
Mon, Aug 3
|
|
Epidemiology
The Effect
of Hand Washing on Skin Microbiota
|
|
Tues, Aug 4
|
|
Effects of
Antimicrobials on Microorganisms; Quiz #2
|
|
Wed, Aug 5
|
|
Role of
Temperature in the Growth of Bacteria
The Effect
of heat on Microorganisms
Effects of
UV Light on Microorganisms & DEMO: osmotic pressure
|
|
7
|
Mon, Aug 10
|
|
Yeasts and
Molds
|
|
Tues, Aug 11
|
|
Protozoa;
and DUE DATE FOR: The Bacterial Unknown Report
|
|
Wed, Aug 12
|
|
Lab Review
|
|
8
|
Mon, Aug 17
|
|
Lab Final Exam
|
|
Tues, Aug 18
|
|
|
|
Wed, Aug 19
|
|
Lecture
Final Exam
|
Laboratory Schedule pdf
|